Lei Yang
Liaoning Shihua University, China
Title: Culture and harvest of mesenchymal stem cells on temperature-responsive glass sphere based carriers
Biography
Biography: Lei Yang
Abstract
It is urgent to preserve cell stemness and exploit appropriate techniques of large-scale expansion for in vitro cultivation of stem cells. The development of temperature-responsive tridimensional carriers is expectedly helpful to solve the above problems and provides a large number of high-quality seed cells for biomedical fields. Based on the preparation strategy of thermo-sensitive planar films and bottom-spray coating technology, the thermo-sensitive glass sphere based carriers were fabricated in this study. Firstly, random block copolymers bearing N-isopropylacrylamide, hydroxypropyl methacrylate and 3-trimethoxysilypropyl methacrylate were synthesized by free radical polymerization. The copolymer solution was then uniformly sprayed on the surfaces of glass spheres via a self-made bottom spray fluidized bed reactor, followed by thermal annealing, so as to form the glass-copolymer composite carriers. The composition and surface morphology were preliminarily determined to investigate the coating effect of the smart copolymers on the surfaces of glass spheres. Afterwards, bone marrow mesenchymal stem cells (BMMSCs) were cultured on the thermo-sensitive carriers, and cell attachment and detachment were controlled by means of temperature changes. The effects of cooling treatment and enzymatic hydrolysis on harvested cells were also compared. The results showed that the thermo-sensitive materials could be evenly coated on the surfaces of glass spheres through fluidized bed reactor, and BMMSCs cultured and recovered from the thermo-sensitive carriers could maintain higher stem cell characteristics. This study provides a new idea for the development of culture and harvest system for in vitro amplification of stem cells and has high clinical application value.